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The IBC has developed a new policy titled Use of Replication-Defective Adeno-Associated Viral Vectors in Rodents. This policy addresses the risk of recombinant DNA shed into the bedding of rodents following delivery of recombinant AAV vectors and how that bedding/caging should be handled. Below are the main points from this policy:

  • This policy only applies to replication-defective AAV vectors that the IBC has determined can be handled at BSL-1/ABSL-1.
  • Cages must be changed by the laboratory staff for the first 72 hours post-administration of AAV vectors.
  • Bedding from the first cage change and any additional cage changes taking place in the first 72 hours post AAV vector administration must be disposed of as biohazardous waste.
  • It is the responsibility of the PI/researcher to bring the appropriate biohazardous waste bags and secondary medical waste containers to the location where cage changing will take place.
  • Following 72 hours post AAV vector administration, the cage change label should be removed and DLAM staff may then take over cage changing responsibility.
  • Carcasses and tissues from animals exposed to AAV vectors falling under this policy can be disposed of as nonbiohazardous carcass waste.
  • The associated SOP that should be followed is available at

This policy will also be available at

Please contact the IBC Administrative Staff ( or 310-794-0262) with any questions and concerns.