1. QUICK TIP: Formatting Experiments
For each experiment you add to your protocol, you will be asked to provide details about the experiment (question 4 of the experiment pop-up). You should provide the Committee with a clear description of what any given animal will experience from initiation of the study to euthanasia. We suggest you use bullet points, timelines, tables or a flow chart to clearly outline what happens to an animal as part of the experiment. Question 4 of the experiment pop-up has an embedded toolbox you can use to help format your description. After completing question 4 of the pop-up, please remember to create and add all referenced procedures to question 5 as shown in the screenshots below. If you need assistance with creating your experiments, please contact RATSHelp@research.ucla.edu.
2. QUICK TIP: Crump Imaging Core Procedures
Crump Imaging Core standard procedures now available for use in RATS 2.0.
The following Crump Imaging Core procedures are now available in the Standard Library:
|CRUMP IMAGING CORE: microPET Imaging||Mouse||Imaging|
|CRUMP IMAGING CORE: microPET Imaging||Rat||Imaging|
|CRUMP IMAGING CORE: microCT Imaging||Rat||Imaging|
|CRUMP IMAGING CORE: microCT Imaging||Mouse||Imaging|
|CRUMP IMAGING CORE: Optical Imaging||Mouse||Imaging|
|CRUMP IMAGING CORE: Optical Imaging||Rat||Imaging|
To find the Crump Imaging Core standard procedures, click on IACUC, Standard Library, and under the Procedures tab, you can type "%crump imaging core" into the search bar:
These procedures do not include the use of probes or contrast agents. If your lab will be using probes or contrast agents during imaging, you will need to create a separate Team Substance Administration procedure to accompany the imaging procedure you choose.
To create a Team Substance Administration procedure, first search the Standard Library to locate the probe or contrast agent you intend to use (note: we recommend using a %wildcard search to improve the results of your search). If the substance you seek cannot be located, you will need to create a team substance. You can then create a Substance Administration procedure, in which you will add the substance (from the standard library or the team substance you created). After the procedure is created, add the Substance Administration procedure to your experiment along with the Crump Imaging Core standard procedure.
Thank you to our colleagues in the Crump Imaging Core for their partnership in preparing these procedures for use.
Contact RATSHelp@research.ucla.edu if you have any questions.
3. QUICK TIP: Behavioral Testing Core Procedures
We have added Behavioral Testing Core (BTC) procedures to the Standard Library in RATS 2.0. It is our hope that providing BTC tests as standard procedures will make it easier for those of you who use the BTC to populate your smartform during your first amendment or triennial review (TR) in RATS 2.0.
To find BTC standard procedures, click on IACUC, Standard Library, and under the Procedures tab, you can type "%behavioral testing core" into the search bar. This will give you a list of all of the BTC standard procedures currently available for use (we plan to add more in the near future).
Thanks to our colleagues in the BTC for their partnership in preparing these procedures for use.
4. QUICK TIP: Standard Substance Administration Template Procedures
We have recently added the below standard substance administration template procedures to the Standard Library:
|TEMPLATE: Intravenous (IV) Tail Vein Injection||Mouse||Substance Administration|
|TEMPLATE: Intravenous (IV) Tail Vein Injection||Rat||Substance Administration|
|TEMPLATE: Subcutaneous (SC) Injection||Mouse||Substance Administration|
|TEMPLATE: Subcutaneous (SC) Injection||Rat||Substance Administration|
|TEMPLATE: Intraperitoneal (IP) Injection||Rat||Substance Administration|
|TEMPLATE: Intraperitoneal (IP) Injection||Mouse||Substance Administration|
|TEMPLATE: Intramuscular (IM) Injection||Mouse||Substance Administration|
|TEMPLATE: Intramuscular (IM) Injection||Rat||Substance Administration|
|TEMPLATE: Oral Gavage||Rat||Substance Administration|
|TEMPLATE: Oral Gavage||Mouse||Substance Administration|
|TEMPLATE: Retro-orbital Injection||Rat||Substance Administration|
|TEMPLATE: Retro-orbital Injection||Mouse||Substance Administration|
To use these procedures on your protocols, you will first need to create a copy of the standard template procedure. To create a copy, click on the procedure you want to copy; this will take you to the procedure workspace. In the procedure workspace, click on the "Copy Procedure" button to create your team copy (see screenshot below). In your team copy, you will add the team and/or standard substances that will be administered via that specific route of administration (e.g., SC injection).
You can also add the standard template procedures directly to your experiments; however, with this approach, you will need to create a separate team substance administration procedure that contains all the substance(s) you will be using in your experiments.
We encourage you to take advantage of the standard templates when building your team’s procedure library.
5. QUICK TIP: Standard Library
In RATS 2.0, there are close to 900 standard substances and over 170 standard procedures available to you for use on your protocol(s). It is important that you first search the Standard Library of substances and procedures prior to creating your own team substances and procedures; this will hopefully reduce the amount of substances and procedures you need to create yourself. You can access the Standard Library by clicking on the IACUC tab in RATS 2.0.
We strongly encourage you use the wildcard search, whereby you use the % symbol on either side of part or all of the search term (e.g., %buprenor%) when searching for existing substances and procedures in the library. When searching for standard procedures, it is also highly encouraged that you use filters to refine your search results. For example, you can filter by species to find all standard procedures for a specific species (e.g., mouse). You are able to add multiple filters to your search. For example, you can add a second filter to search for a specific procedure type (e.g., all standard euthanasia procedures for mice) as shown in the screenshot below:
If you find substances you need for your protocol(s) in the Standard Library, you can create a Substance Administration procedure(s) where you will add the standard substances and detail how they are being administered (e.g., via subcutaneous injection). Once created, the Substance Administration procedure(s) can be added directly to the experiments on your protocols.
If you find a procedure you will be performing on your protocol(s) in the Standard Library, you can add it directly to the experiments on your protocol. You are also able to copy a standard procedure if you need to make an edit to the procedure itself (e.g., if you need to change the dose); when copied, the procedure will become a team procedure. To copy a standard procedure, click on the procedure and once in the procedure workspace, you will have the option to Copy Procedure (as shown in the screenshot below). Only create a copy if you need to make edits to the standard procedure; otherwise you can add the standard procedure directly to your protocol. Please remember to review the details of all standard procedures to ensure that they describe processes that are consistent with how you perform work in your laboratory.
If you have any questions about substances and procedures, refer to the RATS 2.0 Help Center (see screenshot below) or contact RATShelp@research.ucla.edu.
6. QUICK TIP: Pop-Up Windows
As you work in the new system, you will notice that RATS 2.0 frequently opens pop-up windows (e.g., when clicking on hyperlinks or when adding new experiment entries on the Experiments page). It can become confusing if you have multiple windows open at the same, therefore, you might prefer to open all links in a new tab as opposed to having the system open new windows. To do this, right-click on a hyperlink and select "Open Link in New Tab" as shown in the screenshot below. Alternatively, hold the Ctrl key (PC) or Command key (Mac) as you click on a hyperlink. By doing this, all pop-ups and hyperlinks will open as new tabs in one window; this may make it easier for you to keep track of all your open RATS 2.0 pages.
7. QUICK TIP: Building Blocks
Before you start updating your protocol in RATS 2.0, you first need to create building blocks, specifically, your substances and procedures. There is a Standard Library of substances and procedures available for you to use (click on the IACUC tab in RATS 2.0 to access the Standard Library). If you cannot find the substances (e.g., medications, drugs, test compounds, etc.) and/or procedures you need for your protocol(s) in the Standard Library, then create your own team substances and procedures. When searching for existing substances, we strongly encourage using a wildcard search, whereby you use the % symbol on either side of part or all of the search term, for example: %buprenor%
To create your team substances and procedures, go to your Research Team workspace, where you will find the "Create Procedure" and "Create Substance" buttons (see screenshot below). The protocol smartform in RATS 2.0 no longer has a Medications and Experimental Drugs section like the protocol form in Legacy RATS did; therefore, all of your substances (standard and/or team) need to be added to a Substance Administration procedure and attached to an Experiment. The building blocks you create can be reused across all of the protocols under your research team, which is great feature of RATS 2.0 and will hopefully reduce administrative burden in the long-run.
Once your building blocks have been created, you can start working on your RATS 2.0 protocol. When creating an experiment, make sure to add all of the standard and/or team procedures associated with that experiment.
If you have any questions about creating your building blocks, refer to the RATS 2.0 Help Center (see screenshot below) or contact RATShelp@research.ucla.edu.
8. QUICK TIP: Assigning a PI Proxy
When you log on to RATS 2.0, you will notice that we have created a research team for you (when you click on "My Inbox," you will find your research team on the left hand side of the page). When we migrated protocols into RATS 2.0, we added all personnel listed on your Legacy RATS protocol(s) as research team members. As such, it is important that you review and update your research team members list to only include personnel that you would like to have access to your research team substances and procedures (research team members also have the ability to create team substances and procedures). Research team members DO NOT have the ability to create and submit protocols on your behalf, however you can assign a PI Proxy (or multiple PI Proxies) to your protocols to allow them to submit and create protocol applications on your behalf. You can find the "Assign PI Proxy" activity in the protocol workspace (please see screenshot below).
9. QUICK TIP: Live Imaging and Functional Evaluation (LIFE) Core Standard Procedures
We have added the below LIFE Core procedures to the Standard Library in RATS 2.0 with the hope that it will make it easier for those of you who use the LIFE Core to populate your smartform during your first amendment or triennial review (TR) in RATS 2.0.
|LIFE CORE: Optodrum Behavioral Testing||Mouse||Behavioral Testing|
|LIFE CORE: Electroretinography||Mouse||Other|
|LIFE CORE: Fluorescein Angiography||Mouse||Imaging|
|LIFE CORE: Fundus Imaging||Mouse||Imaging|
|LIFE CORE: Visually-evoked Cortical Potentials (VECP)||Mouse||Other|
|LIFE CORE: Optical Coherence Tomography||Mouse||Imaging|
To find the LIFE Core standard procedures, click on IACUC, Standard Library, and under the Procedures tab, you can type "%life core" into the search bar. This will give you a list of all of the LIFE Core standard procedures currently available for use.
Page Last Updated: January 20, 2021